Synthesis of gon-5(10)-enes



July 2, 1968 G, A. HUGHES ET Al. 3,391,165

SYNTHESIS oF GoN-5(1o)-ENEs Filed April 7, 1966 2 Sheets-Sheet 1 o en onze N onza S All All .AIl E N WH zumo... N zumu... N o N N n WWY zo rw zo :w rw zo zw 0 ,S M nro zu zo zu .M AL R E NE w OH W DCJT RRIW OE. GHV Y H E NH N HN HHN onza onza omxu omru Il' Illlv lll' O Nrw C Nrw ro Nrw :a Nrw zo mxo zu zu NH E H n N n N n N n N n o :u Nxzo o ...o Nruw o zu Nruv o :o Nruuu o :u *Il e All T 1| N zu/u Nro/u .n-Nxu xozo s NzNSz/ u :u N Nzo\ July 2 1968 G. A. HUGHES ET AL 3,391,165'

SYNTHESIS OF GON --5(lO -ENES Filed April 7, 1966 2 Sheets-Sheet i.

m 52:3 m ge,

MILD Aclo HYoRoLYsls cHso o m SH3 m 943 xl 12H3 l CH2 ou CH2 cocHB CH2 ococn3 kcnso caso o FIG. 4

X (EH-3 xx (|:H3 m HB CH2 o CH2 oH CH2 on c=cH cuacrus caso caso cnso xm CH3 :XXIII CH3 m CH3 l I l CH2 oH CH2 oH CH2 0H cHzcHa cnzcn3 cuzcHS o caso cHso l/vvE/vroRs GORDON A. HUGHES HERCHEL SMITH BY ATTORNEY United States Patent O" 3,391,165 SYNTHESIS F GON-5(10)ENES Gordon Alan Hughes, Haverford, Pa., and Herchel Smith,

500 Chestnut Lane, Wayne, Pa. 19087; said Hughes assignor to said Smith Continuation-impart of application Ser. No. 228,384,

Oct. 4, 1962. This application Apr. 7, 1966, Ser.

11 Claims. (Cl. 260-397.3)

ABSTRACT 0F THE DISCLOSURE The preparation of novel 13-polycarbon-alkylgon (10)enes by hydrolysis of the corresponding 3-alkoxygona2,5(10)diene is described. These compounds have estrogenic and progestational activity, and, in addition, are useful as inter-mediates for the preparation of compounds having progestational, anabolic, and androgenic activities.

This application is a continuation-impart of co-pending application Ser. No. 228,348, filed Oct. 4, 1962; which in turn is a continuation of applications Ser. No. 57,904, filed Sept. 23, 1960; Ser. No. 91,341, filed Feb. 24, 1961; Ser. No. 137,535, filed Sept. l2, 1961; Ser. No. 195,000, filed May 15, 1962; and Ser. No. 196,557, filed May 16, 1962; all abandoned.

This invention relates to compositions of matter classified in the art of chemistry as substituted unsaturated gonane derivatives, and processes for making and using such compositions.

In describing the invention, reference will be made in the following specification to the annexed drawings, where- FIGURE 1 illustrates schematically the reaction sequence for preparing a 13-alkylgon-5(10)ene, specifically 13-ethyl-17ethynyl-17-hydroXygon-5 (10)-en-3- one.

FIGURE 2 illustrates schematically the mild acid hydrolysis of a 13-alkylgona2,5(10)-diene to a 13-a1kylgon- 5 10)-ene, specifically 13 -ethyl-3-methoxygona-2,5 10)- dien-17-one to 13,8-ethylgon-5 (10)ene-3,l7dione.

FIGURE 3 illustrates schematically the reaction sequence for preparing an ester of a 13-alkyl-l7-hydroxygon-S-ene from a 13alkylgona-2,5(10)-dien-,17-01, specifically the acetate ester of l3-ethyl-l7-hydroxygon- 5(10)-en3one from l3ethyl3-methoxygona-2,5(l0)- dien-17-ol.

FIGURE 4 illustrates schematically the reaction sequence for preparing a 13,8,17a-dialky1-l7-hydroxy-gon- 5(l0)-en3-one from a 1318* alkylgonal,3,5(l0),8tetra en-17-one, specifically l3p,17adiethy1-17-hydroxygon- 5 (l\0)`en3one from 13-ethyl-3-methoxygona-1,3,5 (10), 8-tetraen-17-one.

The invention sought to be patented in a principal composition aspect is described as residing in the concept of a gon5(l0)-ene nucleus having attached thereto in the 13-position a monovalent polycarbonalkyl radical.

The tangible embodiments of the composition aspect of the invention possess the inherent general physical properties of being white crystalline solids, are substantially insoluble in water and are generally soluble in polar solvents such as dimethylacetamide. Examination of compounds produced according to the hereinafter de scribed process reveals, upon ultraviolet and infrared spectographic analysis, spectral data supporting the molecular structures herein set forth. The aforementioned physical characteristics, taken together with the nature of the starting materials and the mode of synthesis, confirm the structure of the compositions sought to be patented.

3,391,165 Patented July 2, 1968 ice The tangible embodiments of the invention possess the inherent use characteristics of exerting qualitatively varying hormonal effects in animals as evidenced by pharmacological evaluation according to standard test procedures. Such tangible embodiments show androgenic, antiestrogenic, progestational, blood-lipid effects, and anabolic actions, salt retention, salt excretion and central nervous system effects. This finding indicates their usefulness in the treatment of amenorrhea, dysmenorrhea, ovulation block and contraception, functional uterine bleeding, arteriosclerosis, hormone dependent tumors, infertility, pregnancy maintenance, habitual abortion, weight gain and nitrogen retention, growth stimulation, post operative recovery, healing of Wounds, and healing of burns. In particular, it has been established that alterations of the natural steroid structure lmade possible by our discovery result not merely in a change of degree of hormonal activity, but, as a result of the separation of types of hormonal activity, alter in an unexpected way its basic nature so that a desirable hormone effect is maximized and an undesirable hormone effect is minimized. Furthermore, said tangible embodiments possess the use characteristic of being intermediates for the preparation of compositions exerting hormonal effects as evidenced by standard test proce-dures.

The invention sought to be patented in a subgeneric composition aspect is described as residing in the concept of a l3-alkyl-l7alkynyll7-hydroxygon-5(10)-en-3 one (FIGURE 1, XVI), of which a specific embodiment, 13/3 ethyl-17aethynyll7hydroxygon5(10)-en-3-one, is hereinafter described.

The tangible embodiments of said subgeneric composition aspect possess the use characteristic of varying hormone effects in animals as evidenced -by pharmacological evaluation by standard test procedures.

The invention sought to be patented, in a principal process of making the compositions aspect, is described as residing in the concept of the sequence of rreactions including: converting a compound having a 5-phenylpent-1yne nucleus, ring-unsubstituted in at least one position ortho to the point of chain attachment, by means of a Mannich type reaction, to its acetylenic Iamine derivative; hydrating the acetylenic linkage to form a 3-keto compound; reacting such 3-keto substrate compound with a nucleophilic 2-monovalent alkyl-1,3-dioxocyclopentano compound under Michael condensation conditions to attach the cyclopentane compound through its 2-position carbon atom to the 1-position carbon atom of the 3keto compound; treating the bicyclic triketone formed in the preceding step with an acidic dehydrating agent thereby to effect a double cyclodehydration to form a l,3,5(10),8,14-pentadehydro-l3-alkyl gonane; selectively saturating the 14(15) double bond of said gonane with hydrogen in the presence of a catalyst; thereafter saturating the 8(9) double -bond of the compound resulting from the preceding step; partially reducing the A-ring double bonds and the 17carbonyl group to 17- hydroxymethylene; and -thereafter converting the so-reduced compound to a 5-dehydro-13-alkyl 17 hydroxygonane.

The invention sought to be patented in a second process aspect, as illustrated in annexed FIGURE 2, is described as residing in the concept of a reaction comprising hydrolyzing compound with a gona2,5(l0)diene nucleus, having attached thereto in the 13-position a monovalent polycarbon-alkyl radical (XIV), under mild conditions, i.e. low temperature, weak racid, to obtain a compound with a gon5(10)-en3one nucleus having attached thereto in the 13-position a monovalent polycarbon-alkyl radical (XVII).

The invention sought to be patented in a third process aspect, :as illustrated in annexed FIGURE 3, is described as residing in the concept of a sequence of reactions including: esterifying the hydroxy group of a compound with a gona-2,5(l)dien-l7-ol having attached thereto in the l3-position a monovalent polycarbon-alkyl radical (XIII) to obtain ia compound with a l7-acyloxygona- 2,5 (10)-diene nucleus having attached thereto in the l3- position a monovalent polycarbon-alkyl group (XVIII), and hydrolyzing the enol ether group under mild conditions to obtain the corresponding 5(10)-en3one (XIX).

The invention sought to be patented in a fourth process aspect, as illustrated in annexed FIGURE 4, is described as residing in the concept of a sequence of reactions including; treating a compound with a gona-1,3,5(),8 tetraen-l7-one nucleus having attached thereto in the l3- position a monovalent polycarbon-alkyl radical with an organometallic derivative of a l-alkyne to obtain the corresponding 17a alkynylgona 1,3,5 (l0),8tetraen17ol having attached thereto in lthe 13-position a monovalent -polycarbon-alkyl radical; selectively saturating the alkynyl group with hydrogen in the presence of a catalyst, thereafter saturating the 8(9) double bond of the compound resulting from the previous step; partially reducing the A-ring double bonds; and, thereafter converting the soreduced compound by mild acid hydrolysis to a l7-alkyll7-hydroxygon-5(10)-en-3-one having attached thereto in the 13-position a polycarbon-alkyl radical.

The manner of making the chemical compounds, which are the starting materials for use in making the compounds of the invention, and for use in the processes of making the invention, are illustrated in copending application Serial No. 228,384 filed October 4, 1962.

The manner and process of making and using the invention will now be generally described so as to enable a person skilled in the art of chemistry to make and use same, as follows:

Referring now to FIGURE 1, wherein the compounds are assigned Roman numerals for identication schematically, the sequence of reactions involved in the synthesis of a specific embodiment, namely l3-ethyl-l7-ethynyl- 17-hydroxygon-5(10)-en-3-one, is illustrated 3-(m-methoxyphenyDpropanol (I) is heated with phosphorus tribromide in benzene after dropwise addition in the cold to -form S-(m-methoxyphenyl)-propyl bromide (II). This halogen compound (II) dissolved in tetrahydrofuran is condensed with sodium acetylide in liquid .ammonia to obtain 5-(m-methoxyphenyl)-l-pentyne (III). Compound III is allowed to stand under nitrogen with water, 30% formalin, acetic acid, diethylamine, dioXan and cuprous chloride at 70 C. for about twelve hours whereby there is obtained l-diethylamino-6-(m-methoxyphenyl)-3-hexanone (IV), which is in turn hydrated in the presence of a mercury salt and sulfuric acid to form 1-diethylamino- 6 (rn-methoxyphenyl)-3-hexanone (V). The ketamine (V) may eliminate diethylamine on distillation to give the vinyl ketone 6 (m-methoxyphenyl)-l-hexen-El-one (VI).

Either the ketamine (V) or the ketone (VI), or mixtures thereof, is then reacted with 2-ethyl-1,3cyclopentanedione (VII) under Michael condensation conditions, e.g. refluxing in methanolic potassium hydroxide to form 2 ethyl 2 [6-(m-methoxyphenyl)6-oxohexyl]-1,3cycyopentanedione (VIII). Compound VIII is then cyclodehydrated .at the reflux temperature of a solvent, such as benzene, in the presence of a dehydrating acid, such as p-toluene sulfonic acid, to effect simultaneous ring closures to give the tetracyclic compound 13-ethyl-3-methoxygona-1,3,5(10),8,14pentaenl7one (IX). The 14-unsaturation of ycompound IX is then selectively hydrogenated in the presence of a metal catalyst such 4as 2% palladized calcium carbonate, to form 13-ethyl-3-methoxygona-l,3, 5(10),8tetraenl7-one (X). Reduction of Compound X with sodium borohydride in ethanol gives 13-ethyl-3- methoxygona-l,3,5(l0),8trienl7ol (XI), which is then converted to 13e-ethyl 3 methoXygona-l,3,5(10)-trien- 17/3-ol (XII) by alkali metal reduction in liquid ammonia,

to provide the normal gonane configuration of 9,8-8,l4- 14,13 exocyclic substituents, namely trans-anti-trans.

By alkali metal reduction in liquid ammonia in the presence of a proton donor, such as ethanol (Birch reduction), compound XII is converted to l3ethyl3-methoxy gona-2,5 (10)-dien-17-ol (XIII). Oppenauer oxidation of compound XIII yields 13-ethyl-3-methoxygona-2,5(10)- dien-l7-one (XIV), which is ethynylated at the 17-position with lithium acetylide in dimethylacetamide to give 136 ethyl 17a ethynyl 3-methoxygona-2,5(10)-dienl7/3-ol (XV). By hydrolyzing under mild conditions such as treatment with aqueous oxalic acid or acetic acid compound XV is then converted to 13-ethyl-l7a-ethynyl- 17-hydroxygon-5 10)-en-3-one (XVI).

This compound, 13,8 ethyl 17a ethynyl 17,8 hydroxygon-5(10)en-3one, exhibits pituitary -blocking and progestational activity accompanied by a separation of undesirable hormone effects found in the natural steroids (e.g., minimal estrogenic elfects).

While the hereinbefore described processes produce novel and steroidal-like compounds which have an unnatural substituent at the l3-position, it is apparent that the novel and valuable processes of the invention offer a unique feasible route to the corresponding natural steroids if the nucleophilic compound used in the Michael condensation step is 2-methyl-1,3-cyclopentanedone.

The aromatic ring of the phenylpropanol (FIGURE 1, I) used as starting material for the preparation of the compositions and initial preparations of the invention may have one or more substituents, provided, however, at least one position ortho to the position of propanol-chain attachment is unsubstituted so that cyclodehydration to form a cyclic structure can eventually be elfectuated. To activate such ort" J position for said subsequent ring closure, a para-directing group (referred to electrophilic aromatic substitution) such as hydroxy, acyloxy, alkoxy, amino, alkylamino, or acylamino is a necessary substituent on the aromatic ring. The group may be present initially or may be introduced later but before ring closure, either directly, or by conversion from a meta-directing group such as nitro.

The term para-directing group (referring to electrophilic aromatic substitution) as used herein means an activating group as those hereinbefore listed and which activates all positions on the aromatic nucleus. Thus, if the group is para-directing, as defined above, it can be in a position meta to the ortho position to which ring closure is limited by steric considerations, said position being activated even though another position is more highly actvated. Ring closure could not occur at the said more highly activated position because of the above-mentioned steric limitations.

After the tetracyclic structure has been formed, substituents can be introduced into the aromatic A-ring which are not limited as above; however, if such substituted compound is to undergo a reduction, the group is preferably one not sensitive to reduction. After the A-ring has been reduced, the substituents on said A-ring may -be the same as those originally present, or substituents to which they may be converted, such as ketonic oxygen, dialkoxy, alkylenedioxy, alkylenethioxy, and alkylenedithio; or groups introducible by known processes, such as halogen or alkyl.

For the processes of the invention and except for the limitations expressed in this specification, variations of the substituents on the A-ring of the fully formed tetracyclic structures, or on the intermediates leading thereto, are full equivalents of each other.

The carbon atom to which the phenyl group of the starting propanol (I) is attached can be substituted, as, for example, with an alkyl group, such as methyl or ethyl. The 2-carbon atom of the starting phenyl propano] can also be substituted, as, for example, with an alkyl group, such as methyl and ethyl, and, as such, be unchanged throughout the subsequent synthesis. In the tetracyclic structures of the invention these carbon atoms will appear in the 6 and 7positions respectively.

For the processes of the invention and except for the limitations expressed in this specification, variations of the B-ring on the fully formed tetracyclic structures, or on the intermediates leading thereto, are full equivalents of each other.

In the Michael reaction step, the 3-keto substrate compound can be a 6-phenyl-1-hexen-3-one, or alternatively. a 6-phenyl-3-hexanone having attached to the l-position a group which will eliminate with hydrogen to form a 6-phenyl-l-hexen-3-one under Michael conditions. Thus, a 3-keto compound with a l-dialkylamino substituent or its quaternary salt, a l-halo substituent or a l-hydroxy substituent will react with the nucleophilic compound to form the Michael product. The nucleophilic compound can be a carbocyclic, l,3dione of varying ring size, as for example, a tive-membered ring, a six-membered ring etc., ultimately forming a corresponding tive-membered, a sixmembered, etc., D-ring in the tetracyclic structure. The 1,3-cyclodionemay also contain a hetero atom at positions other than position 2, thereby to provide a heterocyclic D-ring in the tetracyclic structure.

The 1,3-cyclodione may also contain a hetero atom at positions other than position 2, thereby to provide a heterocyclic D-ring in the tetracyclic structure. A cyclic nucleophilic compound can be used in conducting the Michael reaction step and the open-chain of the resulting product thereafter ring-closed to form a cyclic D-ring.

For the processes of the invention, and except for the limitations expressed in this specification, variations of the D-ring on the fully formed tetracyclic structure, or on the intermediates leading thereto, are full equivalents of each other. When the nucleophilic compound is 2- methyl-1,3-cyclopentanedione, the invention provides a unique total synthesis for natural steroids; the hydrogens at the 8-position, 9-position, and l4-position being ,8, a, and a, respectively, as in the natural steroids, Thus such valuable therapeutic substances as l9-nortestosterone are made available from easily obtainable and relatively simple and inexpensive starting materials.

Moreover, by varying the group at the 2-position of the nucleophilic Michael condensation reactant, the invention provides a way to produce compounds resembling the natural steroids save at the l3-position. Thus, by varying the substituent at the 2-position of the 1,3-cyclopentanedione, alkyl groups of varying chain length such as, for example, ethyl, isopropyl, cetyl, etc., may be introduced to form the gonane correspondingly substituted at the l3-position. Further, gonanes may be prepared wherein the l3-position is substituted with any organic radical. Thus, but without limiting the generality of the foregoing, an aralkyl, cycloalkyl, or a polycarbon-alkylene bridge bearing a hydroxy, amino, or alkylamino-substituent can readily be placed in the 13-position, and from such compounds other variations of the 13-position substituent can be prepared, as haloalkyls from hydroxyalkyls, or quaternary salts, amides, alkenyls, etc. from aminoalkyls.

4For the processes of the invention and except for the limitations expressed in this specification, variations at the 1 3position of the fully formed tetracyclic structures or on the intermediates leading thereto are the full equivalents of the claimed Iii-position polycarbonalkyl substituents, having physiological acticity ofthe same type.

In any of the intermediate structures or in the tetracyclic structures of the invention wherein the l7position, or position corresponding thereto in the gonane nucleus, is carbonyl, the carbonyl group can be converted to a group such as hydroxymethylene by lithium aluminum hydride reduction; to acyloxymethylene by esterication of the hydroxymethylene group; to alkoxymethylene by etherication of the hydroxymethylene group; to alkylhydroxymethylene by addition of the appropriate organometallic reagent to the carbonyl; or to alkynylhydroxymethylene by addition of the appropriate alkali metal acetylide in a suitable inert solvent; all in the known manner. The carbonyl group may also be ketalized or thioketalized by treating with the appropriate alcohol or glycol in a suitable inert solvent under acidic conditions, as in the presence of an acid such as sulfuric acid, p-toluene sulfonic acid, or boron triuoride etherate, with heating `Where necessary, according to the known art.

While the tetracyclic compounds in this specification and the appended examples are named to describe the contiguration corresponding to that of the natural steroids, it is to be understood that unless otherwise indicated, the product of each of the given manipulative procedures is a racemic mixture which contains said named compound and its enantiomorph.

Representative formulations embodying specific compositions of the invention follow.

A pharmaceutical tablet for use as an oral progestational agent consists of the following ingredients:

13 ethyl 17a-ethynyl-l7-hydroxygon-5(10)!-eng 3-one 5 Carboxymethylcellulose (viscosity 400 cps.) 15 Lactose powder 25 Redried corn starch 25 Magnesium stearate powder 4 Calcium silicate powder, q.s.

A capsule for use as an oral proges'tational agent contains, 1n encapsulating gelatin, the following ingredients:

Pharmaceutically acceptable carriers: can be either solid or liquid. Solid form preparations include powders, tablets, dispersble granules, capsules, cachets and suppositories. A solid carrier can be one or more substances which may also act as diluents, Ailavoring agents, solubilizers, lubricants, suspending agents, binders, or tablet disintegrating agents: it can also be an encapsulating material. In powders the carrier is a iinely divided solid which is in adrnixture with the nely divided compound. In the tablets the compound is mixed with carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired.

The powders and tablets preferably contain from 5 to 10 to 99% of the active ingredient. Suitable solid carriers are magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methyl cellulose, sodium carboxymethylcellulose, a low melting wax, and cocoa butter. The ter-m preparation is intended to include the formulation of the compound with encapsulating material as carrier providing a capsule in which the compound (with or without other carriers) is surrounded by carrier, which is thus in association with it. Similarly, cachets are included. Tablets, powders, cachets, and capsules can be used for oral administration.

Liquid form preparations include solutions, suspensions, and emulsions. The compounds are insoluble in water, but can be dissolved in aqueous-organic solvent mixtures that are non-toxic in the amounts used. As an example may be mentioned water-propylene glycol solutions for parenteral injection. Liquid preparations can also be formulated in solution in aqueous polyethylene glycol solutions. Aqueous suspension suitable for oral use can be made by dispensing the `finely divided compound in water with ciscous material, natural or synthetic gums,

7 resins, etc., for example, gum arabic, ion-exchange resins, methyl cellulose, sodium carboxymethylcellulose and other well-known suspending agents.

APreferably the pharmaceutical preparation is in unit dosage form. In such form, the preparation is sub-divided in unit doses containing appropriate quantities of the compound: the unit dosage form can be a packaged preparation, the package containing discrete quantities of preparation, for example, packeted powders of vials o1 ampules. The unit dosage form can be a capsule, cachet, or tablet itself, or it can be the appropriate number of any of these in packaged from. The quantity of compound in a unit dose of preparation may be varied or adjusted from 1 mg. to 100 mg. (generally within the range of 2.5 to 25 mg.) according to the particular application and the potency of the active ingredient.

The claimed compositions having physiological activity can be incorporated into pharmaceutical formulations including sustained-release agents.

The following preparations illustrate the manner of making the chemical compounds which are the starting materials for -use in the processes of the invention.

Preparation 1 3 (3 -methoxyphenyl -n-.propyl bromide Cool 3-(3-methoxyphenyl)propan-l-ol (125 g.) in benzene (200 cc.) to and add a solution of phosphorus tribromide (86 g.) in benzene (150 cc.) dropwise so that the temperature of the mixture does not rise above 5. Keep the mixture at 0 for 1 hour and then heat at 60 `for 3 hours. Cool, pour onto ice, dilute with ether and separate the organic layer. Wash the organic solution with 3 N aqueous sodium hydroxide, water and dry. Remove the solvent and distil the residue to obtain the title compound (131 g.), B.P. 146-148/l7 mm. ILDN 1.5497.

CloHlsBrO calculated: C, 52.4; H, 5.7; Br 34.8. Found: C, 54.4; H, 5.7; Br 34.7.

Preparation 2.-S-m-methoxyphenylpent-l-yne Add 3-(3-methoxyphenyl)-n-propyl bromide (14 g.) in tetrahydrofuran (15 cc.) with rapid stirring to a solution of sodium acetylide (from 1.84 g. sodium) in liquid ammonia 125 cc.) in a Dewar ask. Continue stirring for 22 hours, then add ammonium chloride (3 g.) and water (50 cc.). Collect the product with ether and Wash and dry the ethereal solution. Dstil to obtain 5-mmethoxyphenylpent-l-yne (7.1 gr., 66%), B.P. 75-78 C./0.06 mm. Hg.

C12H14O calculated: C, 82.7%; H, 8.1%. Found: C, 82.2%; H, 7.8%.

Preparation 3.-1diethylamino-6-m-methoxyphenylheX-Z-yne Allow 5mmethoxypheny1pent1yne (8 g.) to stand for 12 hours at 70 C. under nitrogen with water (2.5 cc.), trioxan (0.5 g.), 30% formalin (5.5 g.), diethylamine (4 g.), acetic acid 2.75 g.), dioxan (25 cc.) and cuprous chloride (0.13 g.). Make the cooled solution alkaline with aqueous sodium hydroxide and extract with ether; then extract `the ether extract with 10% hydrochloric acid: wash the acid extract with ether, make alkaline with 10% aqueous sodium hydroxide, extract with ether, and then wash and dry the ether extract. Distil to obtain 14liethylamino-6-m-methoxyphenylhex-Z-yne (10.6 g., 88%), B.P. 13G-131 C./ 0.1 mm.

C17H25N calculated C, 78.7%; H, 9.7%. Found: C, 78.9%;H, 9.6%.

Preparation 4.-1-Diethylamino--m-methoxyphenylhexan-3-one and 6-m-methoxyphenylhex-l-en-3-one Add mercuric sulphate (0.45 g.) to a swirled solution of 1-diethylamino 6 m-methoxyphenylhex-Z-yne (8.5 `g.) in concentrated sulphur'ic acid (2.5 cc.) and water (25 cc.). Keep the solution under nitrogen at 75 C.

Cil

for 1 hour, then cool, make basic with 10% aqueous sodium hydroxide, and lter through glass wool to re move mercuric oxide. Extract product with ether and wash the ethereal solution. Remove the solvent to obtain the crude ketoamine 1-diethylamino-6-m-methoxyphenylhexan-3-one, infrared absorption peak at 1710,12. Distil under reduced pressure with partial elimination of diethylamine, to obtain a mixture of the ketoamine 1diethylamino6-m-methoxyphenylhexan-3-one and the the vinyl ketone G-m-methoxyphenylhex 1 en-3-one, (7.1 g., ca. 76%), B.P. 140-145 C./0.1 mm.; infrared absorption peaks at 5.85 and 5.95/1, the ketoamine predominating.

Preparation 5 .--2-( 6-m-methoxyphenyl-3 -oxohexyl) 2-ethylcyclopentane-1,3-dione Reflux a mixture of (5.25 g.) of 1-diethylamino-6-mmethoxyphenylhexan-3-one and 6-m-methoxyphenylhex- 1-en-3-one with 2-ethylcyclopentane-1,3-dione, (3.3 g.) in dry 0.12% methanolic solution of potassium hydroxide for 18 hours. Filter the resulting solution, evaporate to dryness and dissolve the residue in ether. Wash the ether solution with alkali, hydrochloric acid, and water, dry and evaporate to obtain as residue the triketone 2-(6-m methoxyphenyl-3-Oxohexyl)-2-ethylcyclopentane-1,3dione (7.1 g.) as a gum.

Preparation 6.-13 -ethyl-3 -methoxygona-1,3,5( 10) 8,14-pentaen-17-one Preparation 7.-13-ethyl-3-methoxygona-l,3,5(10),8 tetraen-17-one Dissolve 13,8 ethyl 3-methoxygona-l,3,5(10),8,14 pentaen17one (2 g.) in dioxan (50 cc.) containing lRaney nickel (ca. 0.5 g.) of moderate activity and shake with hydrogen until 160 cc., the amount corresponding to one molecular proportion has been absorbed. Recrystallize the isolated product from methanol to obtain the title product (1.2 g.), M.P. 11G-115; ultraviolet absorption peak at 280 mp. (e 13,200).

Add 1313` ethyl-3-methoxygona-1,3,5(10);8-tetraert- 17-one (16.8 g.) to a solution of sodium borohydride (6 g.) in methanol (500 cc.), swirl the mixture which boils spontaneously. When all the material has been radded and the reaction has subsided, add acetic acid (l5 cc.). Reduce the mixture in volume by evaporation of most of the solvent, add water and extract the product with ether. Evaporate the washed and dried extracts to obtain crude crystalline product (16.8 g.), M.P. 102- 105 on recrystallization from acetonitrile.

To 1318 ethyl 3-methoxygonal,3,5(10),8tetraen 17,8-01 (16.8 g.) dissolved in a mixture of aniline (150 cc.) and tetrahydrofuran (50 cc.) add liquid ammonia (400 cc.). Add lithium metal (6.0 g.) gradually in small pieces during 10 minutes, and stir the blue suspension obtained. After 2 hours, -add ammonium chloride (50 g.) to

the reaction mixture until a clear solution is obtained; then add water (600 cc.) and ether-extract the mixture. Evaporate the washed and dried extracts to obtain as residue la crystalline solid. Recrystallize from hexane (300 cc.), to obtain 13-ethyl-3-rnethoxygona-1,3,5(10)- trien-17-ol (14 g.), M.P. 126-130.

Preparation 10.-13-ethyl-3-methoxygona-2,5(10)- dien-17-ol Add 13p ethyl 3 methoxygona-1,3,5(10)trien 17d-ol (0.5 g.) in tetrahydrofuran (50 cc.) to stirred liquid ammonia (150 cc.), followed by lithium foil (0.5 g.) and then add ethanol (6 cc.) during 20 minutes. When the blue color is discharged, add water and work up the product with ether, to yield l3-ethyl-3-methoxygona- 2,5 (10)dien-17ol as a solid (0.47 g.).

Preparation 1 1 .--13-ethyl-S-methoxygona-Z,5 10) dien-17-one Reux a mixture of 13ethyl3-methoxygona-2,5(l0)- dien-l7-ol (0.8 g.) aluminium isopropoxide (0.36 g.), toluene (26 cc.) and cyclohexanone (8 cc.) under nitrogen for 3 hours. Allow the solution to cool under nitrogen, add water (5 cc.) and shake the mixture vigorously. Add anhydrous sodium sulphate (5 g.), shake the mixture again, and then allow to stand for 30 minutes. Filter the solution, combine the filtrate with ether-wash ings of the residue, and evaporate, rst at 30/20 mm., then at 50/ 0.1 mm. to leave as la crystalline solid 13;?- ethyl 3-methoxygona-2,5(10)-dien-17-one; infrared absorption peaks at 5.78, 5.92, 6.011., with no absorption due to hydroxyl.

Add a suspension of lithium aluminium acetylide (obtained by passing excess acetylene through a solution of lithium aluminium hydride (2.0 g.) in tetrahydrofuran (25 cc.) with stirring to 1313 ethyl-3-methoxygona-2, 5(10)-dien-l7-one (0.6 g.) in tetrahydrofuran (5 cc.). After standing for 18 hours, add ether (40 cc.), followed by the careful dropwise addition of Water until effervescence ceases. Add anhydrous mangesium sulphate (10 g.) and lter the solution and evaporate the filtrate under reduced pressure to give l3 ethyl-3-methoxy-l7a-ethynyl-gona-2,5(10)dienl7ol (90.6 g.).

Infrared absorption peaks at 2.80, 3.05, 4.59, 6.00m

The following examples illustrate the manner f using the claimed processes of the invention for the preparation of the claimed compositions of the invention.

Example 1.-13-methyl-17a-hydroXy-D-homogon- 10) -en-3-one Add 13p methyl 3 methoxyDhornogona-2,5(10)- dien-17a-01 (4 g.) to a solution of oxalic acid dihydrate (5 g.) in Water (70 cc.) and methanol (200 cc.) in an atmosphere of nitrogen. Add isopropanol (40 cc.) and stir the mixture for 11/2 hours. Filter the reaction mixture, pour the iiltrate into brine and extract with ether. Wash, dry and evaporate the ethereal solution and recrystallize the residue from ethyl acetate to give 13;?- methyl 17a hydroxy D homogon-5(l0)en-3-one (1.85 g.), M.P. S-110. Infrared labsorption peaks at 2.98, 5.85m

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

To prepare 13p ethyl 2-methoxy-17hydroxygon 5(10) en 3-one treat 13-ethyl-2,3-dimethoxygona- 2,5(10)-dien17,o1 with oxalic acid dinydrate according to the manipulative procedure described above.

To prepare 13 ethyl 1 oxo-17-hydroxygon-3-one treat 13 ethyl 1,3-dimethoxygona 1,5(10)-dieu- 17-ol with oxalic acid dihydrate according to the manipulative procedure described above.

To prepare phenethyl 17,3 hjydroxygon5(l0) en 3 one treat 13-phenethyl-3methoxygona2,5(l0)- dien-17-o1 with oxalic acid dihydrate according to the manipulative procedure described above.

To prepare 13 (3 hydroxypropyl)-17-hydroxygon-5(10) en 3 one treatl3-(3-hydroxypropyD-3- methoxygona-2,5(10)-dien-l7-ol with oxalic acid dihydrate according to the manipulative procedure described above.

To prepare 13(13-dimethylaminopropyl)-1-oxo-17- hydroxygonan-3-one treat 1319'-(3-dimethylaminopropyl) 1,3-dimethoxygona-1(10),3-dien-l7,8-ol with oxalic acid dihydrate according to the manipulative procedure described above.

These compounds have estrogenic activity and are useful as intermediates in the preparation of the hormonal compounds of the invention.

Example 2.-13-ethyl-17a-hydroxy-D-homogon-5( l0)- en-3-one Hydrolyze 13ethyl3-methoxy-D-homogona-Z,5( 10)- dien-17a-ol (5 g.) by the method of the preceding example to obtain 13-ethyl-l7ahydroxy D homogon- 5(10)-en-3-one (3.3 g.), M.P. 11G-115 C.; infrared absorption peaks at 2.85, 5.82n.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 3.--13,8-methyl-17-hydroxygon-5(10)-en-3-one Add a slurry of 13-methyl-3-methoxygona-2,5(l0)- dien-17-ol (27.9 g.) in dioxane (50 cc.) with stirring to a mixture of methanol (2000 cc.) and water (390 cc.) containing oxalic acid dihydrate (37 g.) at room temperature and continue stirring until 10 minutes after a clear solution had been obtained. Add water (5000 cc.) and extract the product withbenzene (1 vol.)-ether (1 vol.). Work up in the usual manner and recrystallize the residue from ethyl acetate to give 13/3-methyl-17-hydroxygon-5(10)-en3one (18.8 g.), M.P. l44-148 C.

CIQHZBOZ calculated: C, 79.1; H, 9.8%. Found: C, 79.6; H, 9.6%.

Infrared absorption peaks at 2.93, 5.86/1.; no ultraviolet absorption in the 200-300 my. region.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 4,-13-ethyl-17-hydroxygon-5(10)-en-3-one Stir l3,6-ethyl-3-methoxygona-2,5(10) dien 17,9 ol (0.35 g.) with a mixture of methanol (30 cc.), water (6 cc.) and oxalic acid dihydrate (0.46 g.) at room temperature for 45 minutes. Add water and extract the solution with ether. Wash the ethereal solution with water, saturated aqueous sodium bicarbonate and brine, dry and evaporate. Recrystallize the residue from ethyl acetate to give 13-ethyl-17hydroxygon5(10) en 3 one, M.P. l47-9; infrared absorption peaks at 2.93, 5.8651..

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 5.-1S-ethyl-l7-hydroxygon-5(10)-en-3-one Hydrolyze l3ethyl3-n-propoxygona-2,5(10) dien- 17-ol (0.35 g.) exactly as described in the previous example to give l3-ethyl-17-hydroxygon-5(10)-en-3-one, M.P. 147-9. f

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 6.--13npropy1-17-hydroxygon- 5( 10)-en-3-one Stir 13-n-propyl-B-methoxygona-2,5( 10)-dien-17 -ol (0.350 g.) with a mixture of methanol (30 cc.), water (6 cc.) and oxalic acid dihydrate (0.46 g.) at room temperature and under nitrogen for 50 min. A clear solution forms after 30 minutes stirring. Work up the product by means of ether and repeatedly crystallize from ethyl acetate to yield 13-n-propy1-17-hydroxygon-5(10)-en3 one; M.P. 127-30; infrared absorption peaks at 2.89, 5851i.

C20H30O2 calculated: C, 79.4%; H, 10.0%. Found: C, 79.3%; H, 10.0%.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 7 .-13 -n-butyl-17-hydroxygon-5( 10)-en-3-one Stir 1S-n-butyl-3-methoxygona-2,5(10)-dien 17,8 ol (4.6 g.) under nitrogen in methanol (354 cc.) containing water (59 cc.) and oxalic acid dhydrate (5.85 g.) for 45 minutes at room temperature. Filter, add water (885 cc.) and extract the mixture with ether-benzene. Evaporate the washed and dried extracts and recrystallize the residue from ether to give 13-n-butyl-l7-hydroxygon- (10)en30ne, M.P. 104-107; infrared absorption peak at 5.86y..

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Add l3-isopropyl-3-methoxygona2,5(10)-dien 17,8- ol (0.09 g.) in ethanol cc.) to a solution of oxalic acid dhydrate (0.2 g.) in water (3 cc.). Keep the mixture at 25 for 40 minutes and then add an excess of saturated aqueous sodium bicarbonate solution. Isolate the product with ether and crystallize by cooling and rubbing with ether to give BIS-isopropyl-17-hydroxygon- 5(10)en3one (0.064 g.) M.P. 60-65; infrared absorption peak at 5.85p.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

To a solution of l3-isobutyl-3-methoxygona-2,5(l0)- dien-l7-ol (1.0 g.) in methanol (91 cc.) add oxalic acid dhydrate (1.39 g.) in water (18.1 cc.). Stir the mixture for 2 hours at room temperature, add water and extract with ether. Wash the ethereal extracts with sodium bicarbonate solution and water, dry and evaporate to dryness under reduced pressure to give a gum (0.6 g.). Crystallize the gum from ethyl acetate and thereafter from cyclohexane to furnish analytically pure l3-isobutyl-17- hydroxygon-S(10)-en-3-one, M.P. 133-135; infrared absorption peaks at 2.92, 5.85p.

C21H32O2 calculated: C, 79.7%; H, 10.1%. Found: C, 80.0%; H, 10.2%.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Add y1 3-ethylJ3-methoxygonaf2,5(10) dien 17 one (1.3 g.) with stirring in a slurry in dioxane (6 cc.) to methanol (100 cc.) containing oxalic acid dhydrate (1.9 g.) and water (20 cc.). Continue for 10 minutes after a clear solution is obtained. Add water (500 cc.) and collect the product with ether.

, Rccrystallize the product from ethyl acetate-ether to obtain IS-ethylgon-S(10)-en3,17dione (9 g.), M.P. 1Z0-126 C. To obtain an analytical specimen recrystallize from the same solvent mixture to M.P. 127128 C.; infrared absorption peaks at 5.76, 5.85p; no selective ultraviolet absorption in the 20G-300,1. region.

C19H26O2 calculated: C, 79.7%; H, 9.15%. Found: C, 79.9%; H, 9.15%.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example l 1.--13-methyl-17aa-ethylnyl-Na-hydroxy- D-homogon-S( 10 -en-3-one Add 1319 methyl-3-methoxy-l7aaethylnylDhomo gona-2,5(10)dien-17a-ol (0.3 g.) in dioxane (l0 cc.) with stirring to methanol (20 cc.) containing oxalic acid dhydrate (0.46 g.) and water (6 cc.). Continue stirring for 2 hours, add water and extract the mixture with ether. Dissolve the product in benzene and chromatograph on Florex to give the title compound; infrared absorption peaks at 2.99, 3.09, 5.86/.t.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

To prepare 13-isopropy1-17a-ethyl-17-hydroxygon-5 (10)-en-34one treat 13-isopropyl-17a-ethyl-3-methoxygona-2,5( 10)-dien-17-ol with oxalic acid dhydrate according to the manipulative procedure described above.

To prepare 13isobutyl17er-methyl-17hydroxygon5 (10)-en3one treat 13-isobutyl-17a-methyl-3-methoxygona-2,5(10)dien-17ol with oxalic acid dhydrate according to the manipulative procedure described above.

To prepare 13p,17a-dimethyl-17-hydroxygon-5(l0)en 3-one treat 13,9,17a-dimethyl 3 methoxygona2,5(l0) dien-17-ol with oxalic acid dhydrate according to the manipulative procedure described above.

To prepare 13,8,17adiethyl2methoxy17-hydroxygon` 5(l0)en3-one treat 1318,17a-diethyl-2,3-dimethoxygona- 2,5( 10)-dien-17/3-ol with oxalic acid dhydrate according to the manipulative procedure described above.

To prepare 13p,17a-diethyl-1-oxo17hydroxygon3- one treat 1313,l7a-diethyl-1,3dimethoxygona-l,3(10)- dien-17-ol with oxalic acid dhydrate according to the manipulative procedure described above.

To prepare IS-phenethyl-17aethyl17hydroxygon5 (10)-en-3-one treat 13/8-phenethyl-17a-ethyl-3-methoxygona2,5(10)dien17ol with oxalic acid dhydrate according to the manipulative procedure described above.

To prepare 13-(3-hydroxypropyl)17a-methyl17hy droxygon-5 10)-en-3-one treat 13-(3-hydroxypropyl) 17a-methy1-3-methoxygona-2,5(10)-dien-17-ol with oxalic acid dhydrate according to the manipulative procedure described above.

To prepare 13p-(3-dimethylaminopropyl)-l7a-ethyl- 17,3 hydroxy-l-methoxygon-S(10)-en-3-one treat 13- (3 dimethylaminopropyl)17-ethyl-3-methoxygona-2,5 (10)-dien-17-ol with oxalic acid dhydrate according to the manipulative procedure described above.

These compounds have estrogenic activity yand are uselful as intermediates for preparing the hormonal compounds of this invention.

Add 13-methyl-3-methoxy-17.aa-ethynyl-D-homogona- 2,5(l0)dienl7aol (3 g.) in dioxane (10 cc.) with stirring to methanol (20 cc.) containing oxalic acid dhydrate (0.45 g.) and water (6 cc.). After stirring for 2 hours, add water and extract the mixture with ether. Evaporate the Washed and dried extracts to obtain the title compound; infrared absorption peaks at 2.93, 5.90/t.

This compound has estrogenic and progestational activity and is useful as an intermediate fo preparing the hormonal compounds of this invention.

Stir 13-ethyl-3-methoxy-17aaethynylDhomogona2,5 (10)-dien-17a-ol (4 g.) with oxalic acid dhydrate (4.5 g.) in water (35 cc.) and methanol (250 cc.) for 30 minutes. Pour the mixture into brine and extract with ether. Evaporate the washed and dried ether extract to 13 dryness .and recrystallize the residue from ethyl acetate to obtain the title compound (1.75 g.), M.P. 164-168 C; infrared absorption peaks at 2.92, 3.06, 5.84n.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invent-ion.

Stir 1313, 17-a-diethyl-3methoxyD-homogona-2,5 10 dien-17a-ol (4 g.) with oxalic acid dihydrate (4.5 g.) in water (35 cc.) and methanol (250 cc.) .for 2 hours. Pour the filtered solution into brine .and extract with ether. Evaporate the washed and dried extracts and recrystallize the residue from ether to obtain the title compound (2.5 g.), M.P. 116-123 C.

This compound has estrogenic activity and is useful as an intermediate lfor preparing the hormonal compounds of this invention.

Example 15.-13-ethyl-17wethynyl-17-hydroxygon-5 (10)-en-3-one Add 1313 ethyl-3-methoxy-17a-ethynylgona-2,5(10)- dienl7ol (9.6 g.) in methanol (30 cc.) to a solution of oxalic acid dihydrate (0.45 g.) in water (6 cc.). After standing at room temperature for 45 minutes, add ether (60 cc.) Iand wash, dry, and evaporate the ether solution. Dissolve the residual gum in benzene cc.) and chromato'graph on an activated fullers earth (50 g.) elute with light petroleum containing first a small and then a gradually increasing proportion of benzene to obtain first a crystalline byproduct, followed by the desired title compound. Recrystallize from a mixture of light petroleum yand ethyl acetate, to yield the pure compound (0.15 g.), M.P. 169-173; infrared absorption peaks at 2.99, 5.86n.

This compound has progestational activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 16.--1 3 18-17 ot-diethyl- 17 -hydroxygon-S 10 en3one St-ir 1318,17ot-diethyl-Smethoxygona2,5 10) -dien-l7- ol (5 g.) in methanol (430 cc.) containing water (87 cc.) and oxalic acid dihydrate (6.6 g.) until the solid has completely dissolved. Isolate the product (4.55 g), M.P. 126-134 C. by means of ether and recrystallize repeatedly from ethyl acetate t0 obtain the title compound; M.P. 142-143 C.; infrared absorption peaks at 2.90, 5.8511.

C21H3202 calculated: C, 79.9%; H, 10.2%. Found: C, 79.6%; H, 10.1%.

This compound has progestational activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 17.--13,8-ethyl-17a-propyny1-l7-hydroxygon- 5 10)-en-3-o11e Suspend 13,3 ethyl 3 methoxy-17a-propynylgona2,5 ()-dien-17-ol (4 g.) in methanol (200 cc.) and water (20 cc.). Add oxalic acid (4 g.) followed by dioxane (100 cc.) and stir the mixture until dissolution is complete and then for a further 20 minutes. Add water and filter the precipitated product, wash with water and dry. Crystallize from an ethyl acetate-hexane mixture to obtain the title compound, M.P. 156-l59 C.; infrared absorption peaks at 4.55, 5.83/1; no selective ultraviolet absorption beyond 220 my..

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 18.-13-ethyl-17a-(2propenyl) -17- hydroxygon-S( 10 -en-3-one Suspend 1313 ethyl-S-methoxy-17a-(2-propenyl)-gona- 2,5(10)dien17ol (1.5 g.) in methanol (50 cc.) and Water (5 cc.). Add oxalic acid (1 g.) followed by dioxane (20 cc.), and stir the mixture until dissolution is complete and then for a further 20 minutes. Add water gradually and lter the precipitated material, Wash with water and dry. Recrystallize from an ethyl acetate-hexane mixture to obtain the title compound (1 g); infrared absorption peaks at 2.94, 6.21, 6.10tt; no selective ultraviolet absorption beyond 220 ma.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Suspend 13 -ethyl-S-methoxy- 17ot- 2-isobutenyl) gona- 2,5( 10)-dien-l7-ol in methanol (50 cc.) and water (5 cc.). Add oxalic acid (1 g.) followed by dioxane (20 cc.), and stir the mixture until dissolution is complete and then for a further 30 minutes. Add water and extract the product with ether. Wash, dry, and evaporate the ethereal solution and recrystallize the residue from ethyl acetatehexane to obtain the title compound; infrared absorption peaks at 2.90, 5.85, 6.10, 115,11.; no selective ultraviolet absorption beyond 220 mit.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 20.-1S-n-propyl-17a-methyl-l7- hydroxygOn-S 10) -en-3-one To 1319 n-propyl3methoxy17a-methylgona2,5 10)- den-l7-ol (0.3 g.) in methanol (30 cc.) add a solution of oxalic acid dihydrate (0.46 g.) in water (6 cc.). After stirring at room temperature for 20 minutes, add is0- propanol (30 cc.) and continue stirring for 80 minutes; work up the product with ether and recrystallize from a mixture of ether and hexane to obtain the title compound (0.2 g.), M.P. 15S-163 C.; infrared absorption peak at 5.90,u; no selective ultraviolet absorption beyond 220 mp.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 21.-1Sen-propyl-17a-ethynyl-17/S- hydroxygon-S (10)-en-3-one To 13,3 n-propyl-3methoxy17-ethynylgona2,5 10)- dien-l7-ol (0.24 g.) in methanol (40 cc.) add a solution of oxalie acid dihydrate (0.58 g.) in water (7.6 cc.) and stir the mixture for minutes at room temperature under nitrogen. Add water, Work up the product with ether and purify the residue obtained by chromatography on fullers earth. Crystallize from cyclohexane and then from ethyl acetate to obtain the title compound (0.037), M.P. 182 C.; infrared absorption peaks at 2.90, 3.08, 5.84pt.

C22H30O2 calculated: C, 80.9%; H. 9.3%. Found: C, 80.5%; H, 8.6%.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Stir 1318 n propyl 3 methoxy-17a-propynylgona2,5 (10)-diene-l7-ol (2.5 g.) for 2 hours in methanol (80 cc.) containing water (10 cc.) oxalic acid dihydrate (1.75 g.) and tetrahydrofuran (60 cc.). Add the mixture to brine and extract the product with ether. Evaporate the washed and dried extracts and recrystallize the residue from ethyl acetate-hexane to afford the title compound, M.P. 147-150 C.

C23H32O2 calculated: C, 81.1%; H, 9.5%. Found: C, 80.8%; H. 9.4%.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Stir 13,3 n butyl-17ethynyl-3-methoxygona-2,5 10)- diene-17-ol (2 g.) in methanol (50 cc.) containing oxalic acid dihydrate (0.9 g.) and water (12 cc.) under nitrogen for 2 hours. Pour the mixture into water and extract with ether. Evaporate the washed and dried extracts to a residue, purify by chromatography on Florcx and recrystallize from ethyl acetate-hexane to give 13-n-butyl-17aethynyl-17-hydroxygon-5(10)-en-3-one (750 mg), M.P. 160-164 C.; infrared absorption peaks at 2.87, 3.08, 5.82u.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 24.--13,8-ethyl-17,17-ethylenedioxygon- 5(10)en-3one Add 13-ethyl-3methoxy-17,17-ethylenedioxygona2,5 (10)-diene (11 g.) in dioxaue (45 cc.) with stirring to oxalic acid dihydrate (12 g.) in methanol (900 cc.) containing water (150 cc.). Stir the mixture for 1 hour and work up. Purify the resulting gum by chromatography on Florex and recrystallize from ethyl acetate-hexane to yield 13-ethyl17,17-ethylenedioxygon-5( 10) -en-3-one (2 g. M.P. 117-123" C.; infrared absorption peaks at 5.80, 9.22, 9.57, 10.8/i.

This compound is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 25.-l 7-chloroethynyl-l7-hydroxy-l 3(3- propylgon-S(10)-en-3-one Dissolve 3 methoxy 13-propylgona-2,5(10)-dien-l7- one (8 g.) in ether (250 ml.) and treat with chloroethynyl lithium prepared from 100 ml. of methyl lithium (5.53%, 0.35 M) and cis-dichloroethylene (0.175 M) in ether. Stir the reaction mixture for 20 hours under nitrogen and Work up to obtain 8 g. of a gum (about 50% reaction). Treat with hot methanol (225 ml.) and chill mixture to obtain 17-(chloroethynyl)-3-methoxy-13-propylgona2,5 (10)dienl7-ol (2.5 g.). Hydrolyze 17-(chloroethynyl) 3-methoxy l3 propylgona-2.5( l0)-dien-l7/3ol (l g.) with oxalic acid. 21120 l g., water (l ml.), and methanol (50 mL). Let reaction mixture stand for 2 hours. Dilute reaction mixture with water and isolate the product with ether. Chromatograph on silica gel to obtain title compound (350 mg.), M.P. 174176 C. (Found: C, 73.46; H, 8.37. C22H29O2Cl requires: C, 73.21; H, 8.10%.)

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 26.-13-ethyl-17-(2-hydroxyethoxy) gon- 10 -en-3-one Add an ethereal solution of lithium aluminum hydride (15 rnl. of a 1 M solution) to aluminum chloride 13.3 g. in ether (100; ml.) and stir for 20 min. Add to a cool solution of 13-ethyl-3methoxygona-1,3,5 10)-trien- 17one, cyclic ethylene ketal (17.1 g.) in ether (1000 mi.) and stir the mixture for 4 hours, keeping the reaction mixture cool in an ice-water bath. Dilute with 2 N sulphuric acid until a clear solution results. Separate the ether layer, wash with saturated sodium bicarbonate solution, dry `and evaporate. Treat the residue with ethanol (200 ml.), concentrated hydrochloric acid (5 ml.) and water ml.) on a steam bath for 30 min. Evaporate, then recrystallize from ethanol to obtain l3-ethyl-3- methoxy 17,8 (2 hydr0xyethoxy)gona 1,3,5(10) triene (8.1 g.), M.P.131-132".

Dissolve 13,8-ethyl-3-methoxy-17-(2 hydroxyethoxy) gona1,3,5(10)triene (4.0 g.) in 60 ml. of l-methoxy-2- propanol, 120 ml. of tetrahydrofuran, and 300 ml. of liquid ammonia. Add lithium (4.0 g.) gradually, while stirring, during the course of l hr. Add 8.0 g. of ammonium chloride and water. Filter the resulting precipitate, wash with water, and dry. Dissolve the product in 300 ml. of tetrahydrofuran and 300 ml. of liquid ammonia and treat with 4.0 g. of lithium. Stir for 1 hr. Add absolute alcohol, followed by water. Filter` the resulting precipitate and wash with water. Suspend 1.7 g. in methanol ml.), water (20 ml.), and oxalic acid (2.0 g.). Stir under nitrogen for 2 hr. Add water and separate the product with ether. Wash the organic layer with a saturated sodium bicarbonate solution and dry over magnesium sulfate. Evaporate the ether to obtain the title compound as a gum; infrared maxima at 2.95, 5851i. (Found: C, 75.26; H, 9.83. C21H32O3 requires: C, 75.86; H, 9.70%.)

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 27.-1 7a-chloroethynyl-1 3,8-ethyl-17- hydroxygon-S 10)-en-3-one Prepare lithium chloroacetylide by treating lithium methyl (5.33 g.) in ether (100 cc.) with cis-1,2-dichloroethylene (16.9 g.) in an atmosphere of nitrogen and add 13-ethyl-3-methoxygona-2,5(10)-dien 17 one. Stir the mixture for 48 hours at 25, pour into water and isolate with ether. Triturate the solid product with hot methanol and lter the cooled mixture to obtain l7a-chloroethynyl- 13-ethyl3methoxygona2,5(10)-dien 17,8 0l (4.5 g.). Stir this compound (2.7 g.) in dioxan (30 cc.) with a solution of oxalic acid (3.3 g.) in methanol (200 cc.)- water (40 cc.). Add more dioxan (30 cc.) and stir for one hour in an atmosphere of nitrogen. Filter the reaction mixture and dilute the filtrate with water (21.). Filter the product, dissolve it in ether and wash the ethereal solution with brine and dry with sodium sulphate. Evaporate and crystallize the oily residue from etherhexane to obtain the title compound (1.7 g.), M.P. 114.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 28.-1 3-ethylgon-5 10)-ene-3, l 7-diol Dissolve 13-ethyl-l7-hydroxygon-5(l0)en3one (l0 g.) in tetrahydrofuran (250 ml.) and treat with lithium tritertiarybutoxy aluminum hydride (12 g). After 1 6 hr., add water (20 ml.), and filter the precipitate. To the ltrate, add ether-benzene, wash, dry, and evaporate. Recrystallize the crude product from ethanol to obtain the title compound, M.P. 146-148. (Found: C, 77.5; H, 10.4. C19H30O2 requires: C, 77.5; H, 10.36%.)

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example 29.-1 3,8-ethylgon-5( 10) -ene-3,l7,6*diol, diacetate Dissolve 13-ethylgon-5(10)-ene-3,17 diol (l0 g.) in acetic anhydride (50 ml.) and pyridine (50 ml.) and allow the mixture to stand for 16 hr. Remove the solvents under reduced pressure. Dissolve the residue in etherbenzene. Wash, dry, and evaporate. Recrystallize the residue from hexane to obtain the title compound (10 g.), M.P. 127129. (Found: C, 73.87; H, 9.11. C23H34O4requires: C, 73.76; H, 9.15%.)

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Retiux 3methoxy13 propylgona-1,3,5(10)trien-17- ol (5 g.) with sodium hydride (3.93 g., 50% in oil) in xylene (100 ml.) for 1 hour. Add ethyl iodide (14.3 ml.), reflux for 3 hours, and then allow to stand at 25 for 16 hours. Add water and 2 N hydrochloric acid to pH l, separate the organic layer and extract the aqueous with ether. Wash, dry, and evaporate the combined organic solution and recrystallize the residue from methanol to give l7-ethoxy-3-methoxy 13 propylgona-1,3,5(10) triene (2.9 g.), M.P. 119-121. Dissolve the product (2.2 g.) in 1-methoxypropan-2-ol (60 ml.) and tetrahydrofuran (110l m1.) and stir with liquid ammonia (190 m1). Add lithium (2.2 g.) and when the blue color is discharged, add water and extract with ether. Wash, dry, and evaporate the ethereal extracts to give 17 ,B-ethoxy-3-meth oxy-13propylgona-2,5(10)-diene as a solid.

lCombine 17-ethoxy-3-methoxy-13 propylgona2,5 (10)-diene (1.7 g.) with oxalic acid (1.7 g.) in Water (25 m1.) and methanol (170 mL). Stir the mixture under nitrogen for 1.5 hr. Filter off the small amount of residue, add water to the filtrate, and extract with ether. Wash the ether with bicarbonate and Water, and dry over magnesium sulfate. Evaporate the solvent on a rotary evaporator, leaving 2.7 g. of residue. Dissolve in 25 ml. of hot methanol, reduce the volume, cool in a Dry lce-acetor1e bath, lter, and dry in vacuo over diphosphorus pentoxide to obtain the title compound (500i mg), MP. 110- 115.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Add methyl lithium (94.6 g., .218 mole) in diethyl ether and dilute with ether (200 mL). Cool to Add cis-dichloroethylene (11.0 g., .109 mole) over one hour. Suspend l3ethyl-D-homo3-methoxygona2,5 10)-dien17a* one (12.0 g.) in ether (250 ml.) and add to the reaction mixture. Stir .at room temperature. Cool the reaction in anice bath and add saturated aqueous ammonium chloride (250 ml.) dropwise. Separate the ether layer and Wash with Water until neutral. Dry, remove the solvent, and triturate the crystalline residue with boiling methanol (100 ml.). Dissolve 2.5 g. in tetrahydrofuran (20 mL), and add to methanol (80 ml.)l containing Water (l0 ml.) and (COOH)2.H2O (1.75 g.). Add tetrahydrofuran (20 ml.) and stir under nitrogen for 45 min. to effect solution. Pour the reaction mixture into brine, extract with ether, wash the ether layer, dry, and evaporate. Recrystallize the residue to obtain the title compound (1.40 g), M.P. 15G-154; infrared maxima (potassium bromide) at 2.87, 4.53, 5.84m.

This compound has estrogenic activity and is useful as an intermediate for preparing the hormonal compounds of this invention.

Example .32.-13,8-ethy1-17,17ethylenedioxy3,3

dimethoxygon-S 10 -ene Cool 13 ethyl-17,17-ethylenedioxy 3 methoxygona- 2,5(10)diene (3 g.) in methanol (60 cc.) and ether (45 cc.) to 5, add toluene-p-sulfonic acid (50` mg.) and stir the suspension for 1 hour, when the solid will gradually dissolve and then reprecipitate. Add 5% `aqueous sodium hydroxide and collect the product with ether-benzene. Wash, dry and evaporate the organic solution, and recrystallize from hexaneether containing a trace of pyridine to obtain the title compound (2.6 g.), M.P. 115117.

Example 33.-13-e'thyl-17/3-acetoxy-3amethoxygona- 2,5 )-diene Dissolve 13-ethyl3methoxygona 2,5 (10)-diene-17- ol (1.0 g.) in pyridine (6 cc.) and acetic anhydride (2 cc.) and stir the mixture at for 24 hours. Add ether and wash with 5% aqueous sodium carbonate, water and dry. Remove the solvent under reduced pressure and recrystallize the residue from ether to obtain the title compound 18 (0.65 g.), MP. 14S-146; infrared absorption peaks at 5.77, 5.9, 6.011

Example 34.-1S13-ethyl-17-acetoxygon-5(10)-en-3-one Stir 13-ethyl17acetoxy 3 methoxygona-2,5(10) diene (0.65 g.) in methanol (30 cc.) with oxalic acid (0.5 g.) in water (7 cc.) at 25 for 90 minutes. Add ether (150 cc.) and wash with aqueous sodium bicarbonate, Water and dry. Evaporate the solvent and crystallize the residue from methanol to obtain the title compound (0.53 g.), Ml. 112; infrared absorption peaks at 5.76, 585g.

The subject matter which the applicants regard as their invention is particularly pointed out and distinctly claimed as follows:

1. A chemical compound having the structure whereln R is an alkyl group containing from 2 to 16 carbon atoms;

X is keto, hydroxymethylene, or group convertible thereto by hydrolysis;

Y is keto, or

or group convertible thereto by hydrolysis and R is hydrogen, alkyl, .alkenyl, alkynyl, or haloalkynyl.

2. A compound according to claim 1 having the 13- polycarbon alkyl17-alkynyl-l7-hydroxygon-5 (10)-en-3- one structure.

3. The compound according to claim 1, 13-ethy1-17- hydroxygon-S (10)-en-3-one.

4. The compound according to claim 1, 13-n-propyl l7-hydroxygon-5(10)-en-3one.

6. The compound according to claim 1, 13,8-17a-diethy1-17-hydroxygen-5 (10)-ene-3-one.

7. The compound according to claim 2, 13/8-n-propyl- 17a-ethynyl-17-hydroxygon-5 10) -en-3-one.

8. The compound according to claim 2, 17a-chloroethynyl-17hydroxy1S-n-propylgon-S (10)-en-3-0ne.

9. The compound according to claim 2, 17a-chloroethynyl-13,8-ethyl-17-hydroxygon-5( 10) -en-3-one.

10. The compound according to claim 2, 13-ethyll7a-propynyl-17-hydroxygon5(l0)-en3one.

111. The compound according to claim 1, 13,8-ethylgon- 5( 10)-ene3,17dione.

References Cited Applezweig,vSteroid Drugs, vol. 1, p. 441 and 442, McGraw-Hill, New York.

Applezweig, Steroid Drugs, vol. 2, p. 123.

HENRY FRENCH, Primary Examiner.. 

